Isolation and Characterization of Two Main Sub-component Proteins of Wool
نویسنده
چکیده
Treatment of wool with aqueous alkaline thioglycollic acid converts 65% of it into soluble fragments, which are insoluble at pH 4.6 and aggregate spontaneously and irreversibly on storage. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate shows that the soluble protein consists mainly of two components. The calibration curves for molecular weightsjelectrophoretic mobilities are presented. The main components from the plasmolysed wool have molecular weights 44,000 and 58,000 daltons. Their amino acid compositions have been determined; electron microscopy shows that they form fibrous aggregates.
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